[1] London, M. & Häusser, M. Dendritic computation. Annual Review of Neuroscience 28, 503-532 (2005). doi: 10.1146/annurev.neuro.28.061604.135703
[2] Stuart, G. J. & Spruston, N. Dendritic integration: 60 years of progress. Nature Neuroscience 18, 1713-1721 (2015). doi: 10.1038/nn.4157
[3] Spruston, N. et al. Activity-dependent action potential invasion and calcium influx into hippocampal CA1 dendrites. Science 268, 297-300 (1995). doi: 10.1126/science.7716524
[4] Chen, T. W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295-300 (2013). doi: 10.1038/nature12354
[5] Stosiek, C. et al. In vivo two-photon calcium imaging of neuronal networks. Proceedings of the National Academy of Sciences of the United States of America 100, 7319-7324 (2003).
[6] Grienberger, C. & Konnerth, A. Imaging calcium in neurons. Neuron 73, 862-885 (2012). doi: 10.1016/j.neuron.2012.02.011
[7] Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73-76 (1990). doi: 10.1126/science.2321027
[8] Svoboda, K. & Yasuda, R. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50, 823-839 (2006). doi: 10.1016/j.neuron.2006.05.019
[9] Yuste, R. & Denk, W. Dendritic spines as basic functional units of neuronal integration. Nature 375, 682-684 (1995). doi: 10.1038/375682a0
[10] Svoboda, K., Tank, D. W. & Denk, W. Direct measurement of coupling between dendritic spines and shafts. Science 272, 716-719 (1996). doi: 10.1126/science.272.5262.716
[11] Svoboda, K. et al. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385, 161-165 (1997).
[12] Duemani Reddy, G. et al. Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity. Nature Neuroscience 11, 713-720 (2008). doi: 10.1038/nn.2116
[13] Katona, G. et al. Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes. Nature Methods 9, 201-208 (2012). doi: 10.1038/nmeth.1851
[14] Nadella, K. M. N. S. et al. Random-access scanning microscopy for 3D imaging in awake behaving animals. Nature Methods 13, 1001-1004 (2016). doi: 10.1038/nmeth.4033
[15] Gonzalez, K. C. et al. Movement-stabilized three-dimensional optical recordings of membrane potential changes and calcium dynamics in hippocampal CA1 dendrites. Neuron 114, 1818-1832.e9 (2026). doi: 10.1016/j.neuron.2026.01.004
[16] Lu, R. W. et al. Video-rate volumetric functional imaging of the brain at synaptic resolution. Nature Neuroscience 20, 620-628 (2017). doi: 10.1038/nn.4516
[17] Lu, R. W. et al. Rapid mesoscale volumetric imaging of neural activity with synaptic resolution. Nature Methods 17, 291-294 (2020). doi: 10.1038/s41592-020-0760-9
[18] Chen, W. et al. High-throughput volumetric mapping of synaptic transmission. Nature Methods 21, 1298-1305 (2024). doi: 10.1038/s41592-024-02309-3
[19] Kazemipour, A. et al. Kilohertz frame-rate two-photon tomography. Nature Methods 16, 778-786 (2019). doi: 10.1038/s41592-019-0493-9
[20] Cheng, A. et al. Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing. Nature Methods 8, 139-142 (2011). doi: 10.1038/nmeth.1552
[21] Demas, J. et al. High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy. Nature Methods 18, 1103-1111 (2021). doi: 10.1038/s41592-021-01239-8
[22] Mok, A. T. et al. A large field-of-view, single-cell-resolution two- and three-photon microscope for deep and wide imaging. eLight 4, 20 (2024). doi: 10.1186/s43593-024-00076-4
[23] Zhang, Z. X. et al. High-speed neural imaging with multiplexed miniaturized two-photon microscopy. Cell Reports Methods 5, 101221 (2025). doi: 10.1016/j.crmeth.2025.101221
[24] Xu, D. L. et al. Two-photon 3D imaging of optically stimulated neural activity at 100 Hz. Light: Science & Applications 15, 303 (2026). doi: 10.1038/s41377-026-02395-2
[25] Hou, Y. W. et al. Multi-resolution analysis enables fidelity-ensured deconvolution for fluorescence microscopy. eLight 4, 14 (2024). doi: 10.1186/s43593-024-00073-7
[26] Cao, R. J. et al. Dark-based optical sectioning assists background removal in fluorescence microscopy. Nature Methods 22, 1299-1310 (2025). doi: 10.1038/s41592-025-02667-6
[27] Bouchard, M. B. et al. Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms. Nature Photonics 9, 113-119 (2015). doi: 10.1038/nphoton.2014.323
[28] Voleti, V. et al. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nature Methods 16, 1054-1062 (2019).
[29] Nikolenko, V. SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators. Frontiers in Neural Circuits 2, 5 (2008). doi: 10.3389/neuro.04.005.2008
[30] Papagiakoumou, E. et al. Scanless two-photon excitation of channelrhodopsin-2. Nature Methods 7, 848-854 (2010). doi: 10.1038/nmeth.1505
[31] Packer, A. M. et al. Two-photon optogenetics of dendritic spines and neural circuits. Nature Methods 9, 1202-1205 (2012). doi: 10.1038/nmeth.2249
[32] Yang, W. J. et al. Simultaneous two-photon imaging and two-photon optogenetics of cortical circuits in three dimensions. eLife 7, e32671 (2018). doi: 10.7554/eLife.32671