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Application of fluorescence lifetime imaging-integrated deep learning analysis for cancer research
Vibha Kamath, Vyasraj G Bhat, Gagan Raju, Yury V. Kistenev, Nirmal Mazumder
Published Published online: 05 August 2025 , doi: 10.37188/lam.2025.049
Fluorescence lifetime imaging microscopy (FLIM) has emerged as a transformative imaging technique in cancer research, offering quantitative insights into cellular metabolism, tumor microenvironments, and therapeutic responses. By measuring the fluorescence lifetimes of metabolic cofactors such as NADH and FAD, FLIM facilitates the analysis of cancer-specific metabolic reprogramming and heterogeneity. Integration with deep learning further enhances FLIM’s diagnostic and therapeutic potential, enabling high-resolution imaging, automated data analysis, and biomarker identification. This review provides a comprehensive overview of the principles and technological advancements of FLIM, highlighting its applications in cancer diagnostics, drug delivery, and therapy, as well as its integration with deep learning to increase imaging precision and data interpretation. Challenges such as high costs, high computational complexity, and the need for standardized imaging protocols are also addressed. By bridging FLIM with cutting-edge computational techniques, this review highlights its potential to revolutionize cancer research, paving the way for early diagnosis, personalized therapies, and deeper insights into tumor biology.
Overview of advanced optical manufacturing techniques applied in regulating laser damage precursors in nonlinear functional KHxD2-xPO4 crystal
Jian Cheng, Guang Chen, Mingjun Chen, Linjie Zhao, Qiao Xu, et al.
Published Published online: 30 July 2025 , doi: 10.37188/lam.2025.048
Nonlinear KHxD2-xPO4 crystal optics, e.g. second/third-harmonic generators, are components of high-energy/power laser facilities, which deliver and convert 1ω, 2ω, and 3ω lasers to obtain extreme fusion ignition conditions (high pressures, high temperatures, etc.). A laser facility requires extremely high-precision and defect-free KHxD2-xPO4 optics with meter-sized apertures to control laser beams temporally, spatially, and spectrally, yielding great ultra-precision manufacturing challenges. Meanwhile, when irradiated by intense laser pulses, laser damage precursors (e.g. manufacturing-induced micro-cracks, scratches, and debris) in the optics would spark off laser-induced surface damage and damage growth, which have been the bottleneck problems preventing the promotion of the output energies of these laser facilities. Under this circumstance, a variety of advanced optical manufacturing techniques have been developed to regulate these precursors to improve the laser damage resistance of the optics. However, the damage thresholds (8–9 J/cm2) of these optics are still far below the intrinsic threshold of the KHxD2-xPO4 (147–200 J/cm2). Furthermore, the batch engineering applications of these techniques remains challenged by the meter-sized apertures of the optics and their soft-brittle, easily deliquescent, anisotropic, and temperature-sensitive material properties, among others. This work summarises the development of state-of-the-art advanced manufacturing techniques and their problems applied in regulating laser damage precursors in the functional KHxD2-xPO4 optics. Because of their soft-brittle, deliquescent, anisotropic nature, etc., these crystal optics are difficult to cut, and new damage precursors (i.e. corrosion, debris, tool marks) could be introduced in the manufacturing processes. The challenges and their solutions are emphatically discussed and analysed in this paper. The latest development trends for the manufacture of high-performance KHxD2-xPO4 optics with high laser damage resistance are also explored. This work could provide basis and guidance for the function-oriented high-performance manufacturing of KHxD2-xPO4 optics and other functional optics with similar material properties, advancing the development of high-energy/power laser facilities.
Industrial applications of AR headsets: a review of the devices and experience
Artem B. Solomashenko, Olga L. Afanaseva, Maria V. Shishova, Igor. E. Gulianskii, Sergey. A. Sobolnikov, et al.
Published Published online: 21 April 2025 , doi: 10.37188/lam.2025.023

This review considers the modern industrial applications of augmented reality headsets. It draws upon a synthesis of information from open sources and press releases of companies, as well as the first-hand experiences of industry representatives. Furthermore, the research incorporates insights from both profile events and in-depth discussions with skilled professionals. A specific focus is placed on the ergonomic characteristics of headsets: image quality, user-friendliness, etc. To provide an objective evaluation of the various headsets, a metric has been proposed which is dependent on the specific application case. This enables a comprehensive comparison of the various devices in terms of their quantitative characteristics, which is of particular importance for the formation of a rapidly developing industry.

Non-destructive optical measurement of transparent objects: a review
Hongda Quan, Wenqi Shi, Lingbao Kong
Published Published online: 11 April 2025 , doi: 10.37188/lam.2025.022

Transparent objects are widely used in various fields, leading to increasing demand for methods of measuring them. However, the measurement of such objects has always been challenging owing to the intricate refraction and reflection phenomena they exhibit. Given that traditional contact measurement methods can damage transparent objects, the use of non-destructive measurement techniques, particularly those based on optical principles, is considered preferable. As a result, various non-destructive measurement methods have been developed for transparent objects by leveraging the unique characteristics of light, and a comprehensive review is imperative for exploring these innovative methods and their potential applications. This review accordingly begins by elucidating the necessity of measuring transparent objects and exploring the concept of transparency. Next, an overview of various non-destructive optical measurement techniques spanning macro-, micro-, and general-scale applications is presented, followed by a discussion of their respective advantages and limitations. Finally, the paper concludes by outlining future directions for potential advancements in the field. This review is expected to serve as a valuable resource for newcomers in the field of transparent object measurement and assist researchers seeking to integrate these techniques into interdisciplinary studies.

Metal and non-metal doped carbon dots: properties and applications
Runnan Yu, Miaoning Ou, Qirui Hou, Changxiao Li, Songnan Qu, et al.
Published Published online: 22 October 2024 , doi: 10.37188/lam.2024.041

Carbon dots (CDs) have shown great potential for application in optoelectronics, owing to their merits of tunable fluorescence, biocompatibility, low toxicity, and solution processability. However, the intrinsic nature of CDs makes them prone to fluorescence quenching in the aggregated state. In addition, the emission peak width at half maximum of a single CD is usually greater than 60 nm, and the emission spectra may exhibit a multi-peak superposition state, resulting in poor monochromaticity. Further, the unsatisfactory quantum yield of CDs restricts their further application. Considering this, doping strategies have successfully improved the electrical, optical, and chemical properties of CDs. The intrinsic structure and electron distribution of CDs can be effectively adjusted by metal or nonmetal doping. Doping atoms generate n- or p-type charge carriers, changing the bandgap energy, and thereby improving the photophysical properties of the CDs. In this comprehensive review, we explore the intricate effects of various doping strategies on CDs and systematically categorize them. Notably, we elaborate on the diverse types of doped CDs and emphasize their photophysical properties, aiming to elucidate the fundamental mechanisms underlying the influence of doping on CD performance. Specifically, this review describes the extensive applications of doped carbon dots (X-CDs) in optoelectronic devices, information encryption, anti-counterfeiting measures, imaging techniques, and detection fields, to spur further X-CD exploration and application.

A comprehensive review of dwell time optimization methods in computer-controlled optical surfacing
Tianyi Wang, Xiaolong Ke, Lei Huang, Qingqing Cui, Zili Zhang, et al.
Published Published online: 08 August 2024 , doi: 10.37188/lam.2024.021

Dwell time plays a vital role in determining the accuracy and convergence of the computer-controlled optical surfacing process. However, optimizing dwell time presents a challenge due to its ill-posed nature, resulting in non-unique solutions. To address this issue, several well-known methods have emerged, including the iterative, Bayesian, Fourier transform, and matrix-form methods. Despite their independent development, these methods share common objectives, such as minimizing residual errors, ensuring dwell time's positivity and smoothness, minimizing total processing time, and enabling flexible dwell positions. This paper aims to comprehensively review the existing dwell time optimization methods, explore their interrelationships, provide insights for their effective implementations, evaluate their performances, and ultimately propose a unified dwell time optimization methodology.

Advances in femtosecond laser synthesis and micromachining of halide perovskites
Shijie Du, Fangteng Zhang, Lin Ma
Published Published online: 15 July 2024 , doi: 10.37188/lam.2024.035

Perovskite materials have become a popular research topic because of their unique optical and electrical properties, that enable extensive applications in information storage, lasers, anti-counterfeiting, and planar lenses. However, the success of the application depends on accomplishing high-precision and high-quality perovskite patterning technology. Numerous methods have been proposed for perovskite production, including, a femtosecond laser with an ultrashort pulse width and ultrahigh peak power with unique advantages such as high precision and efficiency, nonlinearity, and excellent material adaptability in perovskite material processing. Furthermore, femtosecond lasers can induce precipitation of perovskite inside glass/crystals, which markedly enhances the stability of perovskite materials and promotes their application and development in various fields. This review introduces perovskite precipitation and processing via femtosecond lasers. The methods involved and advantages of femtosecond-laser-induced perovskite precipitation and patterning are systematically summarized. The review also provides an outlook for further optimization and improvement of femtosecond laser preparation and processing methods for perovskites, which may offer significant support for future research and applications of perovskite materials.

Meta-device: advanced manufacturing
Borui Leng, Yao Zhang, Din Ping Tsai, Shumin Xiao
Published Published online: 07 March 2024 , doi: 10.37188/lam.2024.005
Metasurfaces are one of the most promising devices to break through the limitations of bulky optical components. By offering a new method of light manipulation based on the light-matter interaction in subwavelength nanostructures, metasurfaces enable the efficient manipulation of the amplitude, phase, polarization, and frequency of light and derive a series of possibilities for important applications. However, one key challenge for the realization of applications for meta-devices is how to fabricate large-scale, uniform nanostructures with high resolution. In this review, we review the state-of-the-art nanofabrication techniques compatible with the manufacture of meta-devices. Maskless lithography, masked lithography, and other nanofabrication techniques are highlighted in detail. We also delve into the constraints and limitations of the current fabrication methods while providing some insights on solutions to overcome these challenges for advanced nanophotonic applications.
Microsphere-assisted quantitative phase microscopy: a review
Vahid Abbasian, Tobias Pahl, Lucie Hüser, Sylvain Lecler, Paul Montgomery, et al.
Published Published online: 15 March 2024 , doi: 10.37188/lam.2024.006

Light microscopes are the most widely used devices in life and material sciences that allow the study of the interaction of light with matter at a resolution better than that of the naked eye. Conventional microscopes translate the spatial differences in the intensity of the reflected or transmitted light from an object to pixel brightness differences in the digital image. However, a phase microscope converts the spatial differences in the phase of the light from or through an object to differences in pixel brightness. Interference microscopy, a phase-based approach, has found application in various disciplines. While interferometry has brought nanometric axial resolution, the lateral resolution in quantitative phase microscopy (QPM) has still remained limited by diffraction, similar to other traditional microscopy systems. Enhancing the resolution has been the subject of intense investigation since the invention of the microscope in the 17th century. During the past decade, microsphere-assisted microscopy (MAM) has emerged as a simple and effective approach to enhance the resolution in light microscopy. MAM can be integrated with QPM for 3D label-free imaging with enhanced resolution. Here, we review the integration of microspheres with coherence scanning interference and digital holographic microscopies, discussing the associated open questions, challenges, and opportunities.

Direct laser writing on halide perovskites: from mechanisms to applications
Yuhang Sheng, Xiaoming Wen, Baohua Jia, Zhixing Gan
Published Published online: 13 March 2024 , doi: 10.37188/lam.2024.004

Metal halide perovskites have emerged as game-changing semiconductor materials in optoelectronics. As an efficient micro-/nano-manufacturing technology, direct laser writing (DLW) has been extensively used to fabricate patterns, micro/nanostructures, and pixel arrays on perovskites to promote their optoelectronic applications. Owing to the unique ionic properties and soft lattices of perovskites, DLW can introduce rich light–matter interactions, including laser ablation, crystallisation, ion migration, phase segregation, photoreaction, and other transitions, which enable diverse functionalities in addition to the intrinsic properties of perovskites. Based on their patterned structures, perovskites have numerous applications in displays, optical information encryption, solar cells, light-emitting diodes, lasers, photodetectors, and planar lenses, which are comprehensively discussed in this review. Finally, we discuss the challenges that must be addressed for the future development of this fascinating field.

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